48.48 dynamic arraytm Search Results


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taqman gene expression assay 10x  (fluidigm)
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fluidigm taqman gene expression assay 10x
FIGURE 1 | HSV1- green fluorescent protein (GFP) infection and response to Poly (I:C) stimulation of healthy and mutant fibroblasts. (A, B) HSV1-GFP infection of healthy and mutant fibroblasts (A) Overlays of the detection of the fluorescence signal in DHF cells (green) infected at 05, 0,1 MOI and non-infected controls with a nuclei Hoechst counterstain (blue). Images were acquired on an Opera QEHS system with a <t>10x</t> objective and correspond to one field of view. Scale bar: 50µm. (B) Quantification of the percentage of infected cells after infection with 0, 0,1 and 0,5 MOIs. Compiled results of the mean percentages presented together for comparison: 2018 (TLR3 +/- cells), Trif (TRIF-/- cells), 1323 (TLR3 +/- cells), Nemo (NEMO -/- cells), TBK1 (TBK1 -/- cells), TLR3-/- cells, control cells C65 et C72, and DHF. Bars plot the mean ± sd of triplicates of each experimental point. All cell lines are SV40 immortalized fibroblasts except DHF which are primary fibroblasts. (C, D) Poly (I:C) stimulation of healthy and mutant fibroblasts (C) Representative images of the immunofluorescence signal of DHF (upper part) and TLR3-/- (lower part) fibroblasts labeled with an anti- NF-kB antibody: non-stimulated cells (left) and stimulated with 10µg Poly (IC). Scale bar: 50µm. (D) Percentage of cells with nuclear NF-kB (activated) for cells stimulated with 1µg and 10µg of Poly (I:C) and control unstimulated cells (0): 2018 (TLR3 +/- cells), Trif (TRIF-/- cells), 1323 (TLR3 +/- cells), Nemo (NEMO -/- cells), TBK1 (TBK1 -/- cells), TLR3-/- cells, control cells c65 et c72, and DHF. Bars plot the mean ± sd of triplicates of each experimental point. All cell lines are SV40 immortalized fibroblasts except DHF which are primary fibroblasts.
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Image Search Results


FIGURE 1 | HSV1- green fluorescent protein (GFP) infection and response to Poly (I:C) stimulation of healthy and mutant fibroblasts. (A, B) HSV1-GFP infection of healthy and mutant fibroblasts (A) Overlays of the detection of the fluorescence signal in DHF cells (green) infected at 05, 0,1 MOI and non-infected controls with a nuclei Hoechst counterstain (blue). Images were acquired on an Opera QEHS system with a 10x objective and correspond to one field of view. Scale bar: 50µm. (B) Quantification of the percentage of infected cells after infection with 0, 0,1 and 0,5 MOIs. Compiled results of the mean percentages presented together for comparison: 2018 (TLR3 +/- cells), Trif (TRIF-/- cells), 1323 (TLR3 +/- cells), Nemo (NEMO -/- cells), TBK1 (TBK1 -/- cells), TLR3-/- cells, control cells C65 et C72, and DHF. Bars plot the mean ± sd of triplicates of each experimental point. All cell lines are SV40 immortalized fibroblasts except DHF which are primary fibroblasts. (C, D) Poly (I:C) stimulation of healthy and mutant fibroblasts (C) Representative images of the immunofluorescence signal of DHF (upper part) and TLR3-/- (lower part) fibroblasts labeled with an anti- NF-kB antibody: non-stimulated cells (left) and stimulated with 10µg Poly (IC). Scale bar: 50µm. (D) Percentage of cells with nuclear NF-kB (activated) for cells stimulated with 1µg and 10µg of Poly (I:C) and control unstimulated cells (0): 2018 (TLR3 +/- cells), Trif (TRIF-/- cells), 1323 (TLR3 +/- cells), Nemo (NEMO -/- cells), TBK1 (TBK1 -/- cells), TLR3-/- cells, control cells c65 et c72, and DHF. Bars plot the mean ± sd of triplicates of each experimental point. All cell lines are SV40 immortalized fibroblasts except DHF which are primary fibroblasts.

Journal: Frontiers in immunology

Article Title: Unveiling Interindividual Variability of Human Fibroblast Innate Immune Response Using Robust Cell-Based Protocols.

doi: 10.3389/fimmu.2020.569331

Figure Lengend Snippet: FIGURE 1 | HSV1- green fluorescent protein (GFP) infection and response to Poly (I:C) stimulation of healthy and mutant fibroblasts. (A, B) HSV1-GFP infection of healthy and mutant fibroblasts (A) Overlays of the detection of the fluorescence signal in DHF cells (green) infected at 05, 0,1 MOI and non-infected controls with a nuclei Hoechst counterstain (blue). Images were acquired on an Opera QEHS system with a 10x objective and correspond to one field of view. Scale bar: 50µm. (B) Quantification of the percentage of infected cells after infection with 0, 0,1 and 0,5 MOIs. Compiled results of the mean percentages presented together for comparison: 2018 (TLR3 +/- cells), Trif (TRIF-/- cells), 1323 (TLR3 +/- cells), Nemo (NEMO -/- cells), TBK1 (TBK1 -/- cells), TLR3-/- cells, control cells C65 et C72, and DHF. Bars plot the mean ± sd of triplicates of each experimental point. All cell lines are SV40 immortalized fibroblasts except DHF which are primary fibroblasts. (C, D) Poly (I:C) stimulation of healthy and mutant fibroblasts (C) Representative images of the immunofluorescence signal of DHF (upper part) and TLR3-/- (lower part) fibroblasts labeled with an anti- NF-kB antibody: non-stimulated cells (left) and stimulated with 10µg Poly (IC). Scale bar: 50µm. (D) Percentage of cells with nuclear NF-kB (activated) for cells stimulated with 1µg and 10µg of Poly (I:C) and control unstimulated cells (0): 2018 (TLR3 +/- cells), Trif (TRIF-/- cells), 1323 (TLR3 +/- cells), Nemo (NEMO -/- cells), TBK1 (TBK1 -/- cells), TLR3-/- cells, control cells c65 et c72, and DHF. Bars plot the mean ± sd of triplicates of each experimental point. All cell lines are SV40 immortalized fibroblasts except DHF which are primary fibroblasts.

Article Snippet: For amplification, 5 μl of each TaqMan Gene expression assay 10X in assay loading reagent (Fluidigm PN100-7611) and 5 μl of each dilution of preamplified cDNA mixed with GE sample loading reagent (Fluidigm PN100-7610) and Taqman Fast advanced Master Mix (Life Technologies, PN4444557) were loaded on the assay and sample inlets of a 48.48 Dynamic ArrayTM IFC for Gene Expression (Fluidigm), after priming of the array, for use in a BioMark HD instrument.

Techniques: Infection, Mutagenesis, Comparison, Control, Labeling